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Immunoassay Optimization Buffers

120 000 - Coating Buffer

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Coating buffer is designed for adsorptive immobilization of proteins and antibodies on plastic surfaces, such as microtiter plates, or other protein binding surfaces. Depending on the protein or the antibody, the pH of the Coating Buffer is crucial for efficient immobilization, as the pH-value can have an influence on the steric structure of proteins or antibodies. For this reason, coating buffer is offered with a pH of 7.4 or 9.6. For the optimization of the coating procedure of a newly developed immunoassay, we strongly recommend testing the two Coating Buffers side by side. Depending on the type of surface and the type of proteins or antibodies, the required incubation time can vary.

Features

  • Intended for adsorptive immobilization of proteins and antibodies on various surfaces
  • Two different pH-values offered (7.4 and 9.6)
  • 10x concentrate

Applications

  • ELISA

Formats

  • 120 125 - Coating Buffer pH 7.4 (based on PBS), 125 mL
  • 120 500 - Coating Buffer pH 7.4 (based on PBS), 500 mL
  • 121 125 - Coating Buffer pH 9.6 (based on carbonate), 125 mL
  • 121 500 - Coating Buffer pH 9.6 (based on carbonate), 500 mL

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