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Immunoassay Optimization Buffers

120 000 - Coating Buffer

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Coating Buffer for ELISA, EIA, RIA, protein arrays and immuno-PCR

Coating Buffer is designed for adsorptive immobilisation of proteins and antibodies on plastic surfaces (for example microtiterplates) or other protein binding surfaces. Depending on the surface as well as on proteins or antibodies the required incubation time can vary. Consequently, individual optimisation of the incubation procedure is recommended.

Depending on the protein or the antibody the pH of the Coating Buffer is crucial for efficient immobilisation. Select from Coating Buffer pH 7,4 and pH 9,6, as the pH-value can have an influence on the steric structure of proteins or antibodies, thus affecting their immobilisation.

For the optimisation of the coating procedure of a newly developed immunoasay we strongly recommend testing the two Coating Buffers side by side.

10x stock solution
Simply dilute with water!