B. megaterium T7 RNA Polymerase High Yield Gene Expression

Bacillus megaterium  high yield T7 gene expression kit,
includes pretransformed protoplasts BMEGT702 (5 x 500µl), pP_T7 cloning vector and pP_T7-GFP control vector (vectors lyophilized, 10 µg each)

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This video shows a growing micro-colony of cells of Bacillus megaterium MS941 expressing GFP observed in a Zeiss Axiovert 200 M microscope. The cells are induced shortly before the film starts. A brightfield image and a fluorescence image are recorded every 5 minutes over a timespan of 5 1/2 hours. For this film the brightfield images are overlayed by the green coloured fluorescence images. Recorded by Stefan Leupold, Institut für Mikrobiologie, Technische Universität Braunschweig.