The Biotin-Capture Beads manufactured by Adar biotech contain modified Avidin that is immobilized on rigid, highly cross-linked beaded agarose possessing high chemical stability. Adar Biotech's modified Avidin provides a substantial improvement over native Avidin. The high affinity to biotin is maintained, while background problems are minimized due to chemical modification on the protein. The Biotin-Capture Beads has proven to be particularly useful in the isolation of antigens and nucleic acids by employing biotinylated antibodies, or nucleic acid probes respectively. Biotin-Capture Beads can be used in numerous additional affinity chromatography applications.

Biotin-Capture Beads Specifications

Matrix: Sepharose CL-4B

Coupling method: TargetLock

Type of Avidin bound to beads: Modified Avidin

Binding capacity: 2-4 mg biotinilated BSA per ml of beads

Bead size: 40-165 μm

Bead structure: Highly cross-linked spherical agarose, 4%

Max back pressure: 0.3 MPa, 3 bar

Max. flow rates: 4 ml/min/cm²

Recommended flow rate: 1-2 ml/min/cm²

Stability of the matrix: pH 2-11.

Storage: 4°C in PBS pH 7.4 added with NaN3 0.05% (w/v) as a preservative.