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DNA Polymerases/SNPase

119600 - AptaHotTaq Polymerase, 5 U/ul


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AptaHotTaq DNA Polymerase

AptaHotTaq is highly suited for qPCR applications. AptaHotTaq minimizes primer-dimer formation, reduces unspecific oligonucleotide priming and therefore, enhances target-specific amplification. AptaHotTaq, which possesses a 5' --> 3' polymerase activity and a 5' flap endonuclease activity is a mixture of the thermostable Taq DNA Polymerase with DNA-aptameres inhibiting the enzyme's activity at temperatures up to 50˚C. At temperatures above 50˚C. the DNA-aptameres reversibly dissociate from the enzyme. The polymerase is activated during normal cycling conditions, allowing for a convenient assembly of PCR reactions at room temperature. AptaHotTaq can be used in typical Taq-based cycling protocols. A separate high temperature incubation step to activate the enzyme is not needed but may help to resolve secondary structures of template DNA that may interfere with the initial amplification cycles. 

5 U/µl

  • qPCR
  • Hot-start PCR
  • High throughput applications
  • Highly active hot-start Taq
  • DNA-aptamere blocked
  • Convenient reaction set-up at room temperature
  • Activation of enzyme at temperature above 50˚C
  • No change or optimation of protocol necessary
Recommended PCR Conditions
Use PCR conditions optimized for Taq DNA Polymerase. In the case of a low amount of DNA template, additional cycles should be used.

Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10mmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72˚C.

Supplied Reaction Buffers

Complete NH 4 Reaction bufferAmmonium buffer with 25 mM MgCl 2
Incomplete NH 4 Reaction bufferAmmonium buffer without MgCl 2
Complete KCl Reaction bufferPotassium buffer 15 mM MgCl 2
IncludedMgCl 2 (100 mM)
We recommend Ammonium buffer for increased yield of PCR products and potassium buffer for increased specificity of PCR. MgCl2 concentration should be optimized for each particular primer-template combination, meanwhile PCR is effective with 2.5mM of MgCl2 in the majority of cases. 

- 20˚C

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