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Anti-Tet-Repressor Antibodies

TET Ab - Anti-Tet-Repressor Antibodies

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We are pleased to offer a set of polyclonal and monoclonal anti-Tet-Repressor (TetR) antibodies, manufactured by MoBiTec. The immunogen is TetR-tetO (Accession no. P04483). These antibodies possess excellent binding properties and have been successfully tested for use in ELISA, Western blot and immunofluorescence assays. They can be used in the detection of Tet-Repressor (TetR), Tet-Repressor fusion protein (TetR-Fusion), tetracycline responsive transactivator (tTA) and reverse tetracycline responsive transactivator (rtTA). We also offer a TET-Repressor Protein as a positive control (see item# TETR1).


TET01 is a polyclonal rabbit anti-Tet-Repressor antibody. It was prepared using TetR-tetO as immunogen, and has been purified on Protein G. It is provided as 200ul of purified antibody, lyophilized. After reconstitution to 200µl in distilled water, it can be further diluted 1:1000 for Western blots and ELISA applications. (Monoclonal antibody TET03 is recommended for immunofluorescence studies.) This antibody has excellent binding properties to TetR. Detection limits with this antibody are as follows: ELISA = 0.2ng; Western Blots = 0.8ng.


TET02 is provided as 1mg of purified antibody, lyophilized. After reconstitution to 100ul in distilled water, it can be further diluted 1:500 - 1:2000 for Western blots and ELISA applications. (Monoclonal antibody TET03 is recommended for immunofluorescence studies.) This antibody has excellent binding properties to TetR. Detection limits with this antibody are as follows: ELISA = 20-50pg; Western Blots = 0.8 - 1ng


TET03 is a mouse monoclonal antibody, IgG1 Kappa. The antibody recognizes amino acid # 37-44 and is ideal for immunofluorescence studies. TET03 is provided as 50µg of purified antibody, lyophilized. After reconstitution to 100µl in distilled water, it can be further diluted 1:100 to 1:500 for immunofluorescence studies.



TET01TET02TET03
TypeRabbit polyclonal IgGmonoclonal IgG1; κ mix monoclonal IgG1; κ
ImmunogenTetR-tetOTetR-tetOTetR-tetO
PurificationAffinity purified via Protein G columnsAffinity purified via Protein A or G columnsAffinity purified via Protein A or G columns
Epitope--TetR:
-Amino acid #84-98
-Amino acid #26-53
TetR:
-Amino acid #37-44
Reconstitution In:200 µl dest. H2O100 µl dest. H2O100 µl dest. H2O
Working dilution for immunofluorescencen.d.n.d.1:100 - 1:500
Working dilution for Western Blots and ELISA1:10001:500 - 1:20001:1000
Detection limit0.2 ng20 - 50 pgn.d.
Detection limit for Western Blot0.8 ng 0.8 - 1.0 ng5 ng


Please note: MoBiTec anti-TetR(B) antibodies were raised against TetR(B)-tetO, but are generally able to detect variants of TetR(B) as well, such as rtTA and its derivatives, whereby TET02 is the most sensitive and promising choice. However, in contrast to TetR(B), rtTA regulator proteins are required and present in cells in much smaller amounts, especially in stable cell lines. Additionally, the regulated gene and its product may downregulate these regulators further, so that it's even more obscured. Detection of rtTA and its derivatives is more difficult than that of TetR(B) and often fails at the level of Western blotting. In such cases, PCR may be used as surrogate due to its sensitivity. Nevertheless, independent references have proven the suitability of MoBiTec's anti-TetR(B) antibodies to detect rtTA. See examples below: 

Therefore, MoBiTec shall not be made responsible or liable for any claims or loss arising from the failure of the anti-TetR(B) antibodies to detect rtTA and its derivatives.

Features

  • Prime tool for studying tet regulatory systems in eukaryotic cells 
  • Suited for ELISA, Western blots and Immunofluorescence (for cells only, not for tissues or tissue slices) 
  • Excellent binding properties

Applications

  • Detection of Tet-Repressor (TetR)
  • Detection of Tet-Repressor fusion protein (TetR-Fusion)
  • Detection of tetracycline responsive transactivator (tTA)
  • Detection of reverse tetracycline responsive transactivator (rtTA) such as rtTA-S or rtTA-M

Contents

  • TET01 - anti tet-repressor, polyclonal rabbit, lyophilized, 3mg
  • TET02 - anti tet-repressor, monoclonal IgG1, mix, lyophilized, 1mg
  • TET03 - anti tet-repressor, monoclonal IgG1, lyophilized, 50 µg

Literature

Publications

Tet Regulatory System








References with Tet-Repressor (TetR) Antibodies