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Anti-Tet-Repressor Antibodies

TET Ab - Anti-Tet-Repressor Antibodies

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Starting at: $293.00

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We are pleased to offer a set of polyclonal and monoclonal anti-Tet-Repressor (TetR) antibodies with excellent binding properties, manufactured by MoBiTec. The immunogen is TetR-tetO (Accession no. P04483 Pubmed)

These Anti-TET antibodies are suitable for the detection of:
  • Tet-Repressor (TetR)
  • Tet-Repressor fusion protein (TetR-Fusion)
  • tetracycline responsive transactivator (tTA)
  • reverse tetracycline responsive transactivator (rtTA) including derivates like rtTA-S or rtTA-M

TET01 is a polyclonal rabbit anti-Tet-Repressor antibody. It was prepared using TetR-tetO as immunogen, and has been purified on Protein G. It is provided as 200ul of purified antibody, lyophilized. After reconstitution to 200ul in distilled water, it can be further diluted 1:1000 for Western blots and ELISA applications. (Monoclonal antibody TET03 is recommended for immunofluorescence studies.) This antibody has excellent binding properties to TetR. Detection limits with this antibody are as follows: ELISA = 0.2ng; Western Blots = 0.8ng.

TET02 is provided as 1mg of purified antibody, lyophilized. After reconstitution to 100ul in distilled water, it can be further diluted 1:500 - 1:2000 for Western blots and ELISA applications. (Monoclonal antibody TET03 is recommended for immunofluorescence studies.) This antibody has excellent binding properties to TetR. Detection limits with this antibody are as follows: ELISA = 20-50pg; Western Blots = 0.8 - 1ng

TET03 is a mouse monoclonal antibody, IgG1 Kappa. The antibody recognizes amino acid # 37-44, and is ideal for immunofluorescence studies. TET03 is provided as 50ug of purified antibody, lyophilized. After reconstitution to 100ul in distilled water, it can be further diluted 1:100 to 1:500 for immunofluorscence studies.

Anti-Tet-Repressor AbsPolyclonal rabbit IgG (TET01)Monoclonal mouse IgG1 cocktail (TET02)Monoclonal Mouse IgG1 (TET03)
Reconstitute in:

200ul dist. H2O

100ul dist H2O

100ul dist H2O

Working dilution for Western blot/ELISA:

1:1000

1:500-1:1000

1:1000

Working dilution for immunofluorescence

n/d

n/d

1:100-1:500

Detection limit in Western blot

0.8ng

0.8-1ng

5ng

Detection limit in ELISA

0.2ng

20-50pg

n/d


Anti-Tet-Repressor Antibodies Information Sheet