Featuring TargetLock conjugation technology
Introduction
Goat Anti-Rabbit IgG (whole molecule) was affinity purified on Rabbit IgG affinity column using IgG isolated from pooled normal Rabbit sera. Eluted antibodies were further purified by Protein G column in order to separate IgGs from other classes of antibodies. Purified Antibodies were coupled to Sepharose Beads using TargetLock Chemistry.
Anti-Rabbit Beads Specifications
Matrix: SepharoseTM CL-4B
Coupling method: TargetLock.
Coupling density: 0.7-1.2 mg/ml of Goat anti Rabbit IgG
Rabbit IgG binding capacity: minimum 0.6 mg/ml of beads
Mean bead size: 40 -165 μm
Range of pH values allowed: 3.0-11.0
Bead structure: Highly cross-linked spherical agarose, 4%
Max back pressure: 0.3 MPa, 3 bar
Max. flow rates: 4 ml/min/cm2
Recommended flow rate: 1-3 ml/min/cm2
Storage: 4°C in PBS pH 7.4, 0.3N NaCl added with 01% (w/v) Azide as a preservative.