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Fusion Protein Purification

AB-PTN01010-01 - Anti-HA Polyclonal Antibody, 500μl


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Anti-HA Polyclonal (chicken IgY isotype) is derived from chicken immunized with a synthetic peptide containing the human Influenza virus Hemagglutinin (HA) conjugated to KLH. Antibodies were purified from eggs using a proprietary method that yields > 90% purity and were further affinity purified over a column conjugated with synthetic HA peptide. The Anti-HA Polyclonal Antibody recognizes the following sequence: -YPYDVPDYA The antibody is suspended in phosphate-buffered saline containing 0.04% sodium azide. The product is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells. It can be used for immunoblotting, ELISA, immunostaining, and immuno-precipitation.

Dilution Factor: A minimum working dilution of 1:500 is determined by immunoblotting, using whole extracts of transfected COS-7 or CHO-K1 cells, expressing HA-tagged fusion protein. In order to obtain the best results in different techniques and preparations we recommend determining the optimal working dilution using a titration test.