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Mag4C Capture and Concentration of Viruses

ACK11000 - Ad MAG-4C Kit (for adenoviruses) 100 captures

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Starting at: $731.00

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Option: Magnetic Separation Rack



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Fast Capture and Concentration of Viruses

Mag4C Kits are specifically developed for capturing, concentrating and storing viruses. These kits are composed of 3 reagents allowing Magnetic Capture/Concentration, Elution and Conservation of viruses.

A Multipurpose Magnetic Separation Rack which accommodates 2 x 50mL, 2 x 15mL and 12 x 1.5mL tubes is also available.

  • Simple, Rapid & Ready-to-use
  • Capture Efficiency: 80-99%
  • Concentration of viruses by magnetic capture in 30-45 minutes
  • High yield of viral capture and recovery
  • Avoids ultracentrifugation, precipitation and chemicals
  • Reduced handling of viruses
  • Suitable for all conditions and viruses
  • Improved virus stability under recommended storage conditions
  • Ideal for cell culture infection (Magnetofection)
  • 2 different kits
    • Mag4C-Ad Kit for adenoviruses
    • Mag4C-Lv Kit for lentiviruses and retroviruses







RESULTS

1/ Capture

Figure 1 : Mag4C beads efficiently capture viral particles. Supernatants (SN) are nearly non infectious (absence of virus) whereas the concentrated virus bound to the Mag4C beads are highly infectious.

2/ Concentration

Figure 2 : After capture, virus complexed to Mag4C beads can be concentrated without losing transduction efficiency.

3/ Elution

Figure 3: Elution is straighforward, easy and very efficient. Mag4C beads are no longer infectious after the elution procedure.


Figure 4: Captured, eluted and concentrated virus is as infectious as untreated virus.  

4/ Storage

Conservation Buffer can be added to eluted viruses or directly to the Mag4C beads-bound viruses for storage.

The Conservation Buffer:
  1. Preserves virus infectivity during storage
  2. Minimizes the loss of viral titers during storage and freeze/thaw cycles
  3. Can be used to conserve viruses bound to Mag4C beads
Figure 5Viruses stored in conservation buffer maintain high infectivity over long-term storage

Magnetic Separation Rack

To ensure the best efficiency of these kits we recommend using the optional Magnetic Separation Rack

The Magnetic Separation Rack is designed for 1.5mL, 15mL, and 50mL tubes. It can accommodate 12 x 1.5mL microtubes, two 15mL tubes or two 50mL tubes. The Magnetic Separation Rack is required for capture, concentration, washing and elution.

   

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