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Antibody & Protein Delivery

AI20000 - Ab-DeliverIN Antibody Delivery Reagent

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Starting at: $221.00

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Ab-DeliverIN™ Transfection Reagent is the first reagent dedicated to the intracellular delivery of fully functional antibodies. This reagent is a lipid-based formulation which forms noncovalent complexes with antibodies. Complexes are internalized by cells and antibodies are released into the cytoplasm without any cytotoxicity. The delivery of antibodies inside living cells represents an alternative to nucleic acids transfection and a powerful strategy for functional studies or therapeutic approaches. This new and innovative reagent opens new fields of investigation in proteomics to elucidate complex molecular mechanisms or to design new potential therapy.


Figure 1: Intracellular delivery of fluorescently labeled IgG into various cells. Fluorescently labeled polyclonal IgG from human serum was delivered into cells seeded in a 24-well plate using 1 μg FITC-IgG and 2 μL Ab-DeliverIN per well. After 24h, cells were fixed and observed under fluorescence microscopy. Left: CHO; middle: BHK-21; right: HEK293.


Figure 2: Time course of intracellular antibody delivery. 1μg of FITC-labeled antibody was delivered into NIH3T3 cells with 2 μl of Ab-DeliverIN in 24-well plates. Cells were collected and fixed with 2% PFA at the indicated time point. The number of fluorescent cells and the mean of fluorescence were determined by cytofluorimetry. The mean fluorescence was used to evaluate the amount of antibody internalized inside cells.

Features

  • Intracellular delivery of functionally active antibodies
  • Can be used with all antibodies
  • Highly efficient in many primary cells and cell lines
  • Serum compatible
  • Biodegradable and non-toxic
  • Straightforward protocol and ready-to-use reagent

Applications

  • Intracellular delivery of antibodies
  • Intracellular localization studies in living cells, protein function with blocking antibodies, protein-protein interaction blocking, FRET studies, etc.

Sizes

  • AI20100 - Ab-DeliverIN™ Transfection Reagent, 100 µl
  • AI20250 - Ab-DeliverIN™ Transfection Reagent, 250 µl
  • AI20500 - Ab-DeliverIN™ Transfection Reagent, 500 µl
  • AI21000 - Ab-DeliverIN™ Transfection Reagent, 1ml