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3D Transfection

TN30000 - 3D-FectIN for 3D Hydrogels

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3D-FectIN for 3D Hydrogels

3D-FectIN™ is the newest reagent specifically developed to directly transfect cells cultured in 3D hydrogels. 3D-FectIN™ is suitable for all kind of hydrogels and cells. This reagent is based on a novel technology that allows adding a third dimension to cell culture.

Other transfection reagents only transfect cells at the surface of 3D structures; only 3D-Fect™ and 3D-FectIN™ promote transfection throughout the 3D cell culture.

3D matrices not only add a third dimension to the cell environment, they also allow creation of significant differences in cellular characteristics and behavior. Because 3D matrices are routinely used in basic research and therapeutic applications, we have developed 3D-FectIN™ transfection reagent specific for hydrogels. In this way, hydrogel based 3D matrices combined with 3D-FectIN™/DNA complexes, are colonized by cells to be transfected in a more natural environment. This method allows studying angiogenesis, tube and acini formation, colonization, neurite growth, tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation etc.

  • Specific for 3D hydrogels
  • Highly efficient on both cell lines and primary cells
  • Efficient with all type of nucleic acids: plasmid DNA, siRNA, shRNA etc
  • Completely biodegradable
  • Serum compatible
  • Long term protein expression

3D-FectIN™ User Manual

3D-FectIN™ transfection reagent - Results

Transfection of various cells on different gels:

cells

Efficient with various hydrogels and matrices:

NameType of Gel
CollagenCollagen-based hydrogels
Collagen-derivedCollagen-derived hydrogels
HAHyaluronic acid
GelatinEctracellular Matrix (ECM)
Fibrin/FibronectinECM
FibrinogeinECM
LamininECM
MatrigelBD Bioscience
Polyethylene GlycolPEGylated hydrogels