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3D Transfection

TN30000 - 3D-FectIN for 3D Hydrogels

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3D-FectIN™ is a 3D Transfection Reagent specifically developed to directly transfect cells cultured in 3D hydrogels. It is suitable for all types of hydrogels and cells. 3D matrices add a third dimension to the cells’ environment and can induce significant differences in cellular phenotype and behavior. Scaffold-based 3D matrices activated with 3D-Fect™/DNA complexes are colonized by cells which are subsequently transfected in situ and in a more natural environment. These transfections can be studied in angiogenesis, tube and acini formation, colonization, neurite growth, tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation, and other areas.


Figure 1: Transfection of various cells on different gels with 3D-fectin.

Figure 2: Collagen-derived hydrogels were loaded with pVectOZ-SEAP complexed with 3D-FectIN™ (1μg / 3 μl) or other transfection reagents according to the manufacturers’ recommendations. 3T3 cells were seeded onto the pre-loaded matrix and alkaline phosphatase (SEAP) secretion was analyzed over 4 days.

Features

  • Highly efficient
  • For 3D Hydrogels
  • Compatible with all types of nucleic acids 
  • Can be used with cell lines and primary cells
  • Completely biodegradable
  • Can be used for long term protein expression
  • Serum Compatible

Applications

  • Transfection of cells growing in 3D-hydrogels

Sizes

  • TN30250 – 3D-FectIN™ 3D transfection reagent, 250 µl (65 transfections)
  • TN30500 – 3D-FectIN™ 3D transfection reagent, 500 µl (125 transfections)
  • TN31000 – 3D-FectIN™ 3D transfection reagent, 1ml (250 transfections)